Antibodies to Plasmodium circumsporozoite protein (CSP) inhibit sporozoite's cell traversal activity

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Monoclonal Antibodies against Plasmodium falciparum Circumsporozoite Protein

Malaria is a mosquito-borne infectious disease caused by the parasite Plasmodium spp. Malaria continues to have a devastating impact on human health. Sporozoites are the infective forms of the parasite inside mosquito salivary glands. Circumsporozoite protein (CSP) is a major and immunodominant protective antigen on the surface of Plasmodium sporozoites. Here, we report a generation of specific...

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Exit of Plasmodium Sporozoites from Oocysts Is an Active Process That Involves the Circumsporozoite Protein

Plasmodium sporozoites develop within oocysts residing in the mosquito midgut. Mature sporozoites exit the oocysts, enter the hemolymph, and invade the salivary glands. The circumsporozoite (CS) protein is the major surface protein of salivary gland and oocyst sporozoites. It is also found on the oocyst plasma membrane and on the inner surface of the oocyst capsule. CS protein contains a conser...

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Monoclonal antibody identifies circumsporozoite protein of Plasmodium malariae and detects a common epitope on Plasmodium brasilianum sporozoites.

We produced a hybridoma secreting an immunoglobulin G1 monoclonal antibody against the circumsporozoite protein of the human malaria parasite Plasmodium malariae (Uganda 1/CDC). The monoclonal antibody produces a circumsporozoite precipitation reaction when incubated with viable sporozoites of P. malariae and reacts at high titers with heat-fixed sporozoites in an indirect immunofluorescent ant...

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The Plasmodium circumsporozoite protein is proteolytically processed during cell invasion

The circumsporozoite protein (CSP) is the major surface protein of Plasmodium sporozoites, the infective stage of malaria. Although CSP has been extensively studied as a malaria vaccine candidate, little is known about its structure. Here, we show that CSP is proteolytically cleaved by a papain family cysteine protease of parasite origin. Our data suggest that the highly conserved region I, fou...

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Although circumsporozoite protein of Plasmodium vivax (Pvcsp) has been considered to be a potential vaccine candidate, there was no information about the sequence variation in CSP of Iranian P. vivax. In current study, the prevalence of two types of Pvcsp was determined by PCR-RPLP in 374 P. vivax infected patients. In north, with one exception, all parasites were VK210, however, in south both ...

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ژورنال

عنوان ژورنال: Journal of Immunological Methods

سال: 2012

ISSN: 0022-1759

DOI: 10.1016/j.jim.2012.01.009